Yes, there is.

[Jonathan de Boyne Pollard]

PRA>         forwarders {
PRA>                 10.0.0.1;
PRA>                 192.168.2.10;
PRA>                 192.168.2.5;
PRA>                 };

All of your forwardees must provide the same view of the DNS namespace 
as one another.  Since one of your forwardees is your Microsoft DNS 
server and the other two are proxy DNS servers provided by your ISP, 
this will not be the case.

Also, unconditional forwarding is *not* the best choice here.  You have 
already divided your DNS services into separate servers.  You might as 
well do things properly and have your Microsoft DNS server provide 
(internal) content DNS service and your ISC DNS server provide proxy DNS 
service.

<URL:http://homepages.tesco.net./~J.deBoynePollard/FGA/dns-server-roles.html>
<URL:http://homepages.tesco.net./~J.deBoynePollard/FGA/dns-monolithic-server-as-proxy.html#BIND>
<URL:http://homepages.tesco.net./~J.deBoynePollard/FGA/dns-monolithic-server-as-content.html#Microsoft>

PRA> 2) and is their any benfit for me to add a reverse lookup zone,

Yes.  But add it on your Microsoft DNS server, not on your ISC DNS 
server.  (This has the added benefit, on top of the benefit of having 
separate content and proxy DNS servers, of integrating better with your 
Microsoft DHCP clients and DHCP server, if you are using them.)  Let 
your Microsoft DNS server, as the content DNS server, publish all of 
your DNS database content.

Similarly, *don't* configure the DNS clients on your workstations to 
talk directly to your Microsoft DNS server.  Configure them to talk only 
to your ISC DNS server.

PRA> if their is how do it do it.

Of all of the mainstream DNS servers, Microsoft's DNS server is the best 
documented.  Most of the "How do I" questions that people ask are 
already covered in Microsoft's own product documentation.  So always 
stop at the product documentation first.

<URL:http://microsoft.com./resources/documentation/WindowsServ/2003/enterprise/proddocs/en-us/sag_DNS_pro_WorkingWithZonesNode.asp>

PRA> 3) hows can i get the DNS get the information for the LAN PC's
PRA> from my windows 2000 server DNS server.

Configure your ISC DNS server to provide "split horizon" DNS service, by 
giving it "stub" "zones" for the relevant portions of the DNS namespace 
whose data are held on your Microsoft DNS server (e.g. the 
"10.in-addr.arpa.", "168.192.in-addr.arpa.", and "local.cfu.com." 
"zones", for starters).  Remove the unconditional forwarding.

<URL:http://homepages.tesco.net./~J.deBoynePollard/FGA/dns-split-horizon.html>

PRA> 4) could this in any way make trouble to the Windows DNS server?

Not if you do things properly.  If, however, you choose the wrong path 
you'll hit problems further along.  For example:  If you unwisely choose 
to put your reverse-lookup "zones" on your ISC DNS server instead of on 
your Microsoft DNS server, you'll hit problems with secure Dynamic DNS 
updates.  For another example:  If you unwisely choose to hold your 
"zones" on both servers, you limit yourself to using only the lowest 
common denominator DNS database replication mechanism, "zone transfer", 
when not doing so would allow you to use other, better, ones instead.

<URL:http://homepages.tesco.net./~J.deBoynePollard/FGA/dns-incompatible-secure-updates.html>